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GtGGT is considered the most halotolerant, as it retains more than 90% of its hydrolytic activity in 4 M NaCl, followed by BsGGT retaining 86% of its hydrolytic activity at 3 M NaCl, while EcGGT lost 90% of its hydrolytic activity at the same salt concentration (Wada et al., 2010

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After two more washes with PBS, biotin was stained with 1 g ml 1 streptavidin-Atto594 (Sigma-Aldrich), and nuclei were stained with NucBlue (Thermo Fisher Scientific, 1:200) in PBS for 60 min at room temperature

doi: 10.1016/j.bcmd.2012.10.006 111 LangloisM

Side effects Despite FDA approval, PHEN/TPM is not approved for obesity pharmacotherapy by the EMA due to cardiovascular safety concerns