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If iron-overloaded patients are not treated with iron chelation therapy, the continuous supply of surplus iron accumulating with each blood transfusion can eventually overload macrophages and spill into the bloodstream in the form of reactive non-transferrin bound iron (NTBI), resulting in irreparable damage to hepatic cells and other critical organs 10

Conditioned medium was collected and recipient HCT116 cells infected adding Polybrene (Merk, #TR-1003-G) (1 g L 1 )

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To check the differences between samples, we have calculated their L 1 distance, defined as the area contained between the spectra (i.e