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Cells plated in glass-bottom 96-well plates were treated for the indicated times and, after fixation with 4% paraformaldehyde, images were acquired using a high-content microscope (Operetta, Perkin-Elmer)

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Article contents Abstract Introduction Allostery: Theoretical framework of conformational behavior Dynamic communication networks and functional specificity Signaling bias not only in GPCRs but also in all dynamic, allosteric proteins GPCRs and dynamic allosteric proteins share signaling principles Conclusions Author contribution Competing interests References Allostery: allosteric networks and allosteric signaling bias Published online by Cambridge University Press: 18 November 2025 Abstract Introduction Allostery: Theoretical framework of conformational behavior Dynamic communication networks and functional specificity Signaling bias not only in GPCRs but also in all dynamic, allosteric proteins GPCRs and dynamic allosteric proteins share signaling principles Conclusions Author contribution Competing interests References Abstract Allosteric communication is established by networks through which strain energy generated at the allosteric site by an allosteric event, such as ligand binding, can propagate to the functional site

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