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We speculated that GGT is involved in tumorigenesis by interfering with glutamine and -KG metabolism, affecting histone methylation of gastric epithelial cells, activating the Wnt signaling pathway, and improving cell proliferation and migration of the infected cells

Clones were screened for disruption of all Zswim8 alleles by amplification of the locus followed by Sanger sequencing of the PCR amplicon

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These limitations often require additional documentation, adding layers of complexity
