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Western Blot Samples were suspended in lysis buffer (250 mM Sucrose, 50 mM Tris pH 7.4, 1 MgCl, and 0.2 % (v/v) Triton X-100)

Nonetheless, clinical trials involving both healthy individuals and those with mild cognitive impairment or AD are warranted to investigate whether melatonin can enhance the glymphatic systems efficacy in reducing brain concentrations of A and tau

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