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Briefly, 10 L of each sample (containing 2 mg/mL total protein) was loaded onto 5% staking and 8% native polyacrylamide gel bathed in 1 Tris-glycine buffer (pH 8.3) and the proteins separated at constant current (120 V) at 4C for 3 hours

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In conclusion, our study demonstrated a new mechanism of TGR5 agonist on inhibiting IEC apoptosis via cAMP/PKA/c-FLIP/JNK signaling pathway in vitro, and highlighted the value of TGR5 agonist as a novel therapeutic strategy for the treatment of UC