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Accumulation of butyryl-carnitine has been associated with abnormal mitochondrial lipid -oxidation in T2D 54 , suggesting a link between TMAO and mitochondrial dysfunction consistent with the finding that TMAO binds to protein kinase R-like endoplasmic reticulum kinase and increases mitochondrial stress 55

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Investigations have demonstrated that most of such detrimental consequences are linked to elevated ROS throughout sperm freezingthawing process

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