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The steps involved in a typical affinity chromatographic separation are as follows, The ligand is first covalently coupled to a matrix, such as agarose beads

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Frequency: Typically every 14 weeks depending on individual needs

erinaceus Mycelium MBP: Myelin basic protein MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide NF200: Neurofilament-200 OL: Oligodendrocyte OPC: Oligodendrocyte precursor cell PFA: Paraformaldehyde SD: SpragueDawley PDL: Poly-D-lysine RRID: Research Resource Identifier (see scicrunch.org) References Bradl, M