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Prior to the detection, the medium was replaced with Seahorse XF Base Medium (102353-100, Agilent, USA), supplemented with either glutamine (2 mM) for the glycolysis stress test or glutamine (2 mM), glucose (10 mM) and pyruvate concentration (1 mM) for the mitochondrial stress test, and subsequently incubated for an additional 30 min at 37 C in a CO 2 -free incubator

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Schellekens D, Reisinger KW, Lenaerts K, Hadfoune M, Olde Damink SW, Buurman WA

In general, your neurotransmitter activity is strongly associated with your ability to appropriately use methyl groups
Gynecologic cancer as a sentinel cancer for women with hereditary nonpolyposis colorectal cancer syndrome