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Second, chromatographic parameters including loading residence time, binding capacity, and wash and elution buffers (Supplementary Figure S1) were investigated to increase the purity of infectious particles from cell culture impurities and empty capsids

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This should also concern an in-depth analysis of the mechanism of action, including NO as the target most affected by the peptide, as well as the off-targets and their possible effects

For the Phase I exploratory cohort, 7.5 ml of whole blood was also obtained for each assay and free DNA was collected using the QIAamp MinElute Virus Spin Kit, according to the manufacturers directions