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Early efforts employed a range of methods to measure AA metabolites, including radioimmunoassay (RIA), enzyme linked immunoassay (ELISA) and spectrophotometry

Glutathione, metabolism and function via the gamma-glutamyl cycle

Hydration is the name of the game

The formation of gamma-glutamylcysteine is the rate-limiting reaction in GSH synthesis and is feedback inhibited by GSH itself, a mechanism responsible for the regulation of cellular GSH concentration

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