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This mixture was incubated for 30 minutes, then acetic acid was added, and the mixture was further incubated in a hot water bath for 3 minutes
doi: 10.1016/0006-2952(88)90176-1

Trends Immunol 39:7079 Corti D, Lanzavecchia A (2013) Broadly neutralizing antiviral antibodies

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consistently identifies altered metabolites in DAMD patients, including phenylalanine, citrate, carnitine, and maltose, which suggests that dysregulation of lipid and carbohydrate metabolism is a key factor in DAMD progression [62]