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In vitro alkaline comet assay For protocol optimization and to gain an initial impression of the genotoxic potential of NAs in the selected liver models (PCLiS, PHH, PRH and HepG2 cells in the presence of rat or hamster S9-mix), the test systems were treated with NDMA as one of the most potent mutagenic and carcinogenic NAs and subjected to the in vitro alkaline comet assay

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