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Annexin-V staining, imaging, and quantification DRG explants seeded on glass bottom dishes (CellVis) were incubated with 1 g/ml Annexin-V (AAT Bioquest) in annexin-V buffer (10 mm HEPES/NaOH, pH7.4, 140 mm NaCl, and 2.5 mm CaCl 2 ) for 15 min at RT
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Even if these concerns are addressed, GBCA administration requires intravenous access, a requirement that places risks on patients and healthcare practitioners, as well as limiting its application in cases when contraindicated (Guo et al., 2018)
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