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Scientists usually wouldnt perform these experiments using these tags, per se, but rather would visualize the protein of interest to see where it localizes or how much of it there is, with and without the localization or degron tag

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However, paracrine non-cell-autonomous effects and mechanisms of neurotoxicity and synaptotoxicity coming from the astrocyte feeder layer are less well defined

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Klauser, A., Strasser, B., Thapa, B., Lazeyras, F