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Protein Purification by HisTrip Column and 6XHis Tag TEV Cleavage Frozen bacterial pellets were resuspended in the lysis buffer (50 mM Tris-HCl, 150 mM NaCl, pH 8.0), and the cells were lysed using an ultrasonic processor
Li N, Zheng D, Xue J, Guo W, Shi J, Sun J, Lu C, Zheng W, Wu M, Cheng S
Age endogenous glutathione production declines with age, mirroring the broader pattern of cellular metabolic slowdown
Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury
Nat Commun 12(1):3644
