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These challenges include sampling (spot urine, 24 h urine, plasma, serum etc.) and analytical issues (missing reference standards, matrix effects, protein adducts, recovery, limit of detection, stability during storage, etc.)

By contrast, the inhibition of the de novo synthesis of GSH, by deleting either the catalytic (Gclc) or the modifier (Gclm) subunit of glutamatecysteine ligase (Gcl), dampens intracellular GSH, increases ROS, and impact T cell differentiation