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Subsequently, an immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany) was used for detection in accordance with the manufacturers instructions

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Importantly, these epigenetic alterations have been shown to persist beyond the period of active androgen exposure, even after partial normalisation of circulating hormone levels

Whereas extracellular cholesterol oxidase is secreted into the broth medium by other genera such as Pseudomonas , Arthrobacter , Rhodococcus equi , Brevibacterium sterolicum , and S
