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Western blotting analysis and enzyme-linked immunosorbent assay (ELISA) assay Whole liver lysates were prepared by sonication in HEPES lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 0.1% Tween 20, 10% glycerol, 0.1 mM Na orthovanadate, 1 mM NaF, 10 mM -glycerophosphate) containing protease inhibitor cocktail (EDTA-free Complete Mini, Roche, Meylan, France)

Drawing on the latest research, this review highlights advanced glycation end products (AGEs), sex hormone-binding globulin (SHBG), and microRNAs (miRNAs) as promising targets for PCOS prevention and treatment
GSH also participates in bile formation and secretion, which is an important part of the metabolism of dietary fats and cholesterol

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