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Infection is rare but possible if the skin or equipment is not clean, which is one of the strongest arguments for having the injection done properly

After washing with TBS containing 0.1% Tween 20, the membranes were incubated for 30 min at 25 C with TBS containing 0.1% Tween 20, 1% BSA, and horseradish peroxidase-conjugated anti-mouse (CST, 7076) or anti-rabbit (CST, 7074) secondary antibodies before visualization using Western Lightning ECL Pro (Perkin Elmer, NEL120001EA)

How long before I can expect noticeable results

doi: 10.1186/s40001-023-01604-7 [DOI] [PMC free article] [PubMed] [Google Scholar] 216.Suda M, Paul KH, Tripathi U, Minamino T, Tchkonia T, Kirkland JL

& Ren, Z