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MD simulation The cell membrane in the simulation system was composed of dioleoylphosphatidylethanolamine (DOPE)dioleoylphosphatidylglycerol (DOPG) lipids with a 3:1 ratio (90 and 30 molecules, respectively)

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HEp-2 cells were infected, and the cells and the supernatant were harvested at 72 hpi, sonicated for 10 min at 35 C, centrifuged, and stored at 80 C to generate rA2-eGFP stocks

Chandra, N

(ii) control (Ctrl) group, microglia (~ 100,000 cells/ml) cultured in complete medium containing 0.4% DMSO for 24 h, then subjected to OGD/R as aforementioned