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Description
Reduction and alkylation of the cysteine residues were then performed with 10 mM DTT and 55 mM iodoacetamide, respectively, followed by adding 360 L of 50 mM ammonium bicarbonate buffer to reduce the urea concentration to below 1 M
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[DOI] [PMC free article] [PubMed] [Google Scholar] 52.Netea M.G., Dominguez-Andres J., Barreiro L.B., Chavakis T., Divangahi M., Fuchs E., Joosten L.A.B., van der Meer J.W.M., Mhlanga M.M., Mulder W.J.M., et al
