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Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline Solubility: 2.75 mg/mL (7.50 mM)

Endocrinology 152:468475 Yakar S, Isaksson O (2016) Regulation of skeletal growth and mineral acquisition by the GH/IGF-1 axis: lessons from mouse models

Sinopoli A, Calogero G, Bartolotta A

In the reaction mixtures without enzyme, varying amounts of MBB (0-300 l of 1 mM stock solution which gave final concentration 0-100 M) were added into different fluorescent cuvettes already containing 3.0 ml of phosphate buffer (pH 6.5) and 100 l of GSH stock solution (3 mM)

Comparative analysis of cell handling techniques in glioblastoma cell lines: Effects on growth and therapeutic response