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Liver sinusoidal endothelial cells

2.1.6 Viability staining Immediately following an 8-hour extravasation/migration experiment, a solution containing 2 g/mL of propidium iodide (5370605ML, Sigma) in EGM-2 was added to the side and top ports of the device and incubated for 10 minutes at room temperature before imaging a 400 m stack in the Z-direction for each lumen, centered at the middle of the lumen

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J.ClarkeG.ONeillA.GroegerJ
