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You might notice mild stinging at the injection site occasionally

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E., Rai, R., Khan, S

For proliferation assays, enriched or sorted B cells were labeled with 5 M CFSE or Celltrace violet (Thermo Scientific) at 37 C for 10 min and then stimulated with anti-IgM, anti-CD40, or LPS for 72 h

Cycling rather than continuous research use is the standard approach