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Staying upright for about 4 hours is a precaution to be extra safe, but if you accidentally lie down for a short time it is unlikely to affect your results

Mitochondrial dysfunction is characterized by various degrees of ultrastructural mitochondrial damage, abnormal morphologic changes, increased permeability of the outer and inner membranes, impaired mitochondrial -oxidation, respiratory chain activity reduction, ROS overproduction, ATP depletion, and oxidative stress-mediated deletions of mtDNA inducing apoptosis (Ramanathan et al

doi: 10.2165/00007256-200636050-00001

Cell Death Dis (2018) 9:119

MazziniLGelatiMProficoDCSorarGFerrariDCopettiMet al