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Instrumentation and analytical conditions Stationary and mobile phase selection Preliminary experiments, including the screening of stationary phases and the selection of an optimal column and mobile phase for the separation of GSH, gGC, and related compounds, were conducted using an Agilent 1260 Infinity II high-performance liquid chromatographic system equipped with a quaternary high-pressure pump, autosampler, column thermostat, and UV diode array detector

800 ng of flag-tagged substrates cloned into pCCF and 200 ng of SelO U667C or SelO U667C D338A pQXCIP was co-transfected into cells using Polyjet (product SL100688, SignaGen) according to the manufacturers instructions