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For preparations made at pH 7.5 which had predominantly diglutathionylated M pro the preparation was incubated at 37C for 30 minutes in the presence of buffer control (50 mM Tris, pH 7.5, 2 mM EDTA, and 100 mM NaCl containing 100 ug/ml of cm-BSA), Grx (350 nM) alone, GSH alone (0.5 mM) and Grx and GSH together