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Acknowledgements This study was supported by the National Research Foundation (NRF) Grant (grant nos
doi: 10.1038/nprot.2007.41
Briefly, mitochondria from BMDM pellets (~10 7 cells) were isolated from the cytosolic fractions after cell permeabilization with a buffer containing 0.1% digitonin in 210 mM mannitol, 20 mM sucrose and 4 mM HEPES
Most of these triggers are enigmatic, but some drugs and viral infections might be involved [13,14,15,16]
Some of them were given exercises to speed up recovery
