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After deparaffinization and rehydration, all sections were treated with microwaves in 10 mmol/L citrate buffer (pH 6.0) for 10 min for antigen retrieval

Samples were then washed once with DBB and twice with PBB

In everyday clinical practice, we increasingly encounter patients who look biochemically older than their chronological age

Also, free radicals can damage sperm DNA through oxidation of purine and pyrimidine bases, breakage of DNA single and double strands, protamine deficiency, chromosomal rearrangement and gene mutation and the detrimental consequences are cell damage, cell apoptosis, and impaired fetus development

It was subsequently shown to have characteristics different from H