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Availability of data and materials The sequence read data of gut microbiome for characterizing TMAO producers (BioProject:PRJNA589723) were all available on NCBI sequence read archive (SRA) database (SRA accession numbers:SRR10465787-SRR10466048)

doi: 10.1001/archinte.160.4.526 72 HollowellJGStaehlingNWFlandersWDHannonWHGunterEWSpencerCAet al

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Some nLDs are associated with tubules in a type I nucleoplasmic reticulum We found several examples of nMFBs that extended parallel to a single-membrane nuclear tubule, or occasionally multiple tubules (arrows, Fig 3H and 3I)