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Two-step assay with fixation prior to chase step: - DBCOcl incubation, formaldehyde fixation, and azido-tagged dye DBCO-labeled cells prepared as described earlier were washed 3X with PBS and resuspended in 4% formaldehyde in PBS and incubated statically at room temperature on the benchtop for 30 min
American Journal of Medical Quality 2015
[3-4] Infections Infectious complications are seen in 0.5-6% of people with a tattoo
In these cases contrast enhancement is not seen, and disruption of the bloodbrain barrier does not occur [73]
