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Activity was monitored via quantification of radiolabeled reaction product histone [methyl- 3 H]-L-arginine

A repeated-measures two-way ANOVA revealed a significant main effect of the factors treatment (F (1,16) = 12.47, P = 0.0028), time (days) (F (5,80) = 4.437, P = 0.0012), and a significant treatment time interaction (F (5,80) = 10.89, P 0.05
This increases cyclic AMP, activates protein kinase A, and stimulates pulsatile GH transcription and release

These changes occurred through direct effects on adipocyte biology rather than appetite suppression, as research models maintained normal feeding behavior while exhibiting significant reductions in individual adipocyte size
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