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In the present investigation, MTX challenge induced ER stress and UPR mainly through the PERK/CHOP pathway, consistent with previous studies on MTX-induced hepatic injury (Schmidt et al., 2022), nephrotoxicity (Song et al., 2021), and cognitive impairment (Lv et al., 2020)

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The combination with hyaluronic acid (6 mg) in the rabbit model produced superior outcomes to either compound alone, which has led some practitioners to explore AOD-9604 in joint health contexts