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This approach has already been explored successfully with TLK286 when combined with pegylated liposomal doxorubicin (Vergote et al., 2010) or with carboplatin plus paclitaxel (Sequist et al., 2009)

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The samples were centrifuged at 1000 rpm for 3 min using a KUBOTA 3520 (KUBOTA, Tokyo, Japan), and absorbance was measured at 595 nm using a GeneQuant 1300 spectrophotometer (Biochrome, Cambridge, UK)

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This consequently results in Nrf2 stabilization, accumulation and translocation into the nucleus where it combines with the corresponding antioxidant-response elements (ARE) switching on the transcription machinery of antioxidant target genes such as heme oxygenase-1 (HO-1), and glutathione reductase (GR) enzymes [22]