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Therefore, an important consideration of this study was to conduct an age-specific sub-analysis

Precisely add 1.0 mL of 0.1% formic acid aqueous solution to the residue, sonicate for 1 min to dissolve the residue, and filter through a 0.22 m membrane for analysis by liquid chromatography-tandem mass spectrometry

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qPCR analysis revealed that lotus leaves upregulated peroxisome proliferator-activated receptor alpha (PPAR-), lipoprotein lipase (LPL), carnitine palmitoyltransferase 1 (CPT1), and cholesterol 7 alpha hydroxylase (CYP7A1) mRNA expressions and downregulated peroxisome proliferator-activated receptor gamma (PPAR-) and CCAAT/enhancer-binding protein alpha (C/EBP-) mRNA expressions, to reduce adipocyte differentiation and fat accumulation, promote oxidation of fat and decomposition of triglyceride and cholesterol