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Melting curve analysis was performed to confirm the production of a single product in each reaction

Subsequently, the undiluted samples were analyzed by LC-MS

The intrinsic ACS activity of FATPs maintains a constant gradient of free fatty acids from the extracellular to the intracellular milieu, with trapping of the long-chain fatty acyl-CoA thioesters inside the cell (vectorial acylation) [17, 20, 2326], a process conserved from Saccharomyces cerevisiae [27]

The muscle weakness experienced by patients with LMNA variants resembles the slower swimming speed and weaker muscle endurance observed in these mutant transgenic fish