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Drug-likeness assessments revealed that the Epothilone-GSH conjugate exhibited more violations of Lipinski's rule of five (3 violations) compared to Epothilone (1 violation), resulting in lower bioavailability scores of 0.11 and 0.55, respectively

In brief, cells were washed twice with phosphate-buffered saline and resuspended in prewarmed (37 C) Seahorse basal medium (Agilent) supplemented with 1 mM pyruvate, 2 mM glutamine, and 5 mM glucose (pH 7.4)

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Ashok V, Dash C, Rohan TE, Sprafka JM, Terry PD (2011) Selective cyclooxygenase-2 (COX-2) inhibitors and breast cancer risk