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HRP was activated using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL) and imaged with a FluorChem Q system (Protein Simple, Wallingford, CT)

For HE staining, tissue sections were deparaffinized in xylene I and II for 10 min each, then sequentially hydrated for 5 min each in xylene/ethanol (1:1), 100% ethanol I/II and 80% ethanol, followed by rinsing with tap water
However, DJ1 deficiency failed to enhance neuronal cell death when challenged with MGO 60

The association of circulating prepartum metabolites, minerals, cytokines, and hormones with postpartum health status in dairy cattle

When using alcohol and distilled water, it can extend the shelf life to several months
