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UCLA Health
Relative expression of target genes was calculated using the 2^(Ct) method ( 2.5 Immunohistochemistry The dewaxed slides (5 m-thick coronal sections) were subjected to antigen retrieval through heat in Tris-EDTA buffer (pH 9.0) at 96C for 40 minutes
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This helps reduce inflammation and speeds up recovery, making movement easier and injuries less persistent over time
