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Cell pellets were collected and lysed in 9 M urea or RIPA buffer (10 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% Na deoxycholate, 0.1% SDS, and 1% Triton X)

The methylcytosine dioxygenase ten-eleven translocase-2 (tet2) enables elevated GnRH gene expression and maintenance of male reproductive function

The generation of oxidative stress could also be used as a therapy

In MASLD treatment, strategies aimed at reducing oxidative stress are gaining importance
