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The ThT kinetic curve is characterized by a lag time ( t lag , the time in which ThT fluorescence reaches 5% of its maximum), dominated by the formation of oligomeric species not detectable by ThT, followed by an elongation phase, in which pre-fibrillar structures elongate to fiber, normally characterized by a time to half ( t 1/2 , the time in which the ThT fluorescence reaches half of its maximum value) and, finally, a plateau region in which the equilibrium is reached and the ThT fluorescence reaches its maximum value I max (Figure S1 in the Supporting Information)

[DOI] [PMC free article] [PubMed] [Google Scholar] 129.Ma D.K., Jang M.-H., Guo J.U., Kitabatake Y., Chang M.-L., Pow-Anpongkul N., Flavell R.A., Lu B., Ming G.-L., Song H

While Vitamin B12 can be obtained from dietary sources such as meat, fish, dairy products, and fortified cereals, some individuals may have difficulty absorbing it due to medical conditions or dietary restrictions