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Storage conditions: The raw materials should be stored away from light in an inert atmosphere at -20C to prevent moisture absorption and chemical degradation

A 2 mL volume of the sample ( V 0 ) was retrieved from the receiver cell after different time intervals, and the cell was supplemented with 2 mL of fresh PBS

Five snapshots of random regions of interest (20X magnification) of the placental villi (trophoblast) were selected for fluorescence intensity quantification using ImageJ software

Potential Pitfalls and How to Avoid Them Even with the best plan, things can go wrong if you're not careful