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All statistical analyses were performed using EZR (Saitama Medical Center, Jichi Medical University, Saitama, Japan) 13 , which is a graphical user interface for R (The R Foundation for Statistical Computing, Vienna, Austria)

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And due to the increasing attentions paid onto the immune therapy and vaccine development, we emphatically discussed the immunological regulation effects of MnO 2 NPs, followed by their promising application in different diseased conditions, which is expected to benefit the future development of novel vaccines and therapies based on MnO 2 NPs