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In dendrites of cortical pyramidal neurons lacking a novel MAM protein PDZ domain-containing protein 8, a significant fraction of Ca 2+ released from the ER during synaptic stimulation accumulates in the cytosol and elevates the local dendritic Ca 2+ levels

Hasriadi, Dasuni WasanaVajragupta PWO, Rojsitthisak P, Towiwat P
Briefly, the residue was reconstituted in 200 L of chloroform:methanol (1:1, v/v) and diluted threefold with isopropanol:acetonitrile:water (2:1:1, v/v/v)