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Free Radic Biol Med 38:14131421
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If you think you will be purifying the same exact protein again in the future, then you should rinse your elution buffer out with a few column volumes of deionized water (dIH 2 O), and then store the beads in 20% ethanol until youre ready to use them again
Always reconstitute and administer each substance separately as directed
Formation of NAPQI adducts on mitochondrial proteins was found to be unique to APAP when compared to its regioisomer 3-hydroxyacetanilide (AMAP), which is non-toxic in mice [24,28,29]
