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Expert Opinion on Therapeutic Patents, 14 (9), 1329-1344

MicroRNAs in fibrosis: opportunities and challenges
If you must freeze reconstituted solutions: Aliquot into single-use portions before freezing Use a standard freezer (-20C), not a frost-free freezer Thaw completely at room temperature before use Never refreeze a thawed solution Use within 1-2 weeks after thawing Frost-free freezers cycle through defrost periods that cause repeated temperature fluctuations

Julsgaard M, Christensen LA, Gibson PR, Gearry RB, Fallingborg J, Hvas CL, et al

The difference is subtle but important: a facilitator helps increase lean mass more directly, whereas an optimizer affects peripheral areas related to the muscle-building process