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Whereas, several studies have demonstrated that high-dose and long-term administration of artemisinin-based drugs has gastrointestinal, neurotoxic, and cardiotoxic effects in experimental animals (e.g., rhesus monkeys, rats, and dogs), and the most common adverse effects are nausea, vomiting, and dizziness (Li et al., 2019

Genes implicated in folate biosynthesis were found across 512 gastrointestinal microbial genomes in the phyla Bacteroidetes , Fusobacteria , Proteobacteria , Actinobacteria

Primers were designed with the help of Oligo Primer Analysis Software version 7 (Molecular Biology Insights, Colorado Springs, CO, USA)

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Pfandl B, M^rike K, Winne D, et al
