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Similarly, oil red O staining of aortic root cross-sections showed a significantly decreased oil red O-positive staining area in the HCD + ALC group compared with that of the HCD group (Figures 5B,C)

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They also mitigate Nf-B overactivated signalling, attenuating the NLRP3 inflammasome pathway and decreasing pro-inflammatory cytokines and PCR levels, thereby showcasing their immunomodulatory activities [161, 202, 217]

Ponceau S protein staining was used on the transfer PVDF membrane to confirm equal protein loading

The significant decrease at 7 days is in agreement with Northrop and Yamamoto 13 , wherein similar decreases in occludin were observed after serial exposure to stress and investigator administered Meth
