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Thereafter, trypsin at 1:50 was poured for overnight digestion and that at 1:100 was introduced for additional 4 h of digestion, followed by peptide desalting using a C18 SPE column

AHK-Cu's histidine residue in the middle position is thought to contribute to particularly stable copper chelation under physiological pH conditions

[DOI] [PubMed] [Google Scholar] 141.Li Y., Li J., Cheng R., Liu H., Zhao Y., Liu Y., Chen Y., Sun Z., Zhai Z., Wu M., et al

51, 74, 75, 76, 77, 78 The molecular mechanisms that underlie the cytotoxic potential of cytoplasmic CDDP are poorly understood, yet may involve: (i) the accumulation of reactive oxygen species (ROS) and nitric oxide (NO), which not only exacerbate CDDP genotoxicity but also exert direct cytotoxic effects by favoring the opening of the so-called permeability transition pore complex (PTPC)

doi: 10.1007/s00535-017-1415-1